5HC3

The structure of esterase Est22

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Lipolytic enzyme	polymer	365	39066.7	4	UniProt (H6BDX1) Pfam (PF07859) In PDB	uncultured bacterium
2	B, D	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3		water	water		18.0	561	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 344 (UniProt: H6BDX1)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 170	Ser 170	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156266.7
Non-Polymers*	Number of molecules	4
	Total formula weight	368.4
All*	Total formula weight	156635.1

*Water molecules are not included.