5H9F

Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target at 2.45 angstrom resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR system Cascade subunit CasA	polymer	502	55965.0	1	UniProt (Q46901) Pfam (PF09481)	Escherichia coli (strain K12)	CRISPR type I-E/Ecoli-associated protein CasA/Cse1,CRISPR-associated protein CasA/Cse1
2	B, C (B, C)	CRISPR system Cascade subunit CasB	polymer	165	19231.0	2	UniProt (P76632) Pfam (PF09485)	Escherichia coli (strain K12)
3	D, E, F, G, H... (D, E, F, G, H...)	CRISPR system Cascade subunit CasC	polymer	363	40071.4	6	UniProt (Q46899) Pfam (PF09344)	Escherichia coli (strain K12)
4	J (J)	CRISPR system Cascade subunit CasD	polymer	224	25239.8	1	UniProt (Q46898) Pfam (PF09704)	Escherichia coli (strain K12)
5	K (L)	crRNA (61-MER)	polymer	61	19677.6	1		Escherichia coli
6	L (M)	DNA (28-MER) Non-target	polymer	28	8598.5	1		Escherichia coli
7	M (N)	DNA (50-MER) Target	polymer	50	15389.9	1		Escherichia coli
8	N (K)	CRISPR system Cascade subunit CasE	polymer	199	22324.0	1	UniProt (Q46897) Pfam (PF08798)	Escherichia coli (strain K12)	CasE endoRNase,crRNA endonuclease
9	O (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
10	AA, BA, CA, P, Q... (M, N, K, A, B...)	water	water		18.0	1050	Chemie (HOH)

Sequence modifications

B, C: 1 - 160 (UniProt: P76632)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Tyr -1	-	expression tag
Gln 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	426085.3
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	426150.8

*Water molecules are not included.