5H75

Crystal structure of the MrsD-Protein A fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Mersacidin decarboxylase,Immunoglobulin G-binding protein A	polymer	238	26267.1	4	UniProt (Q9RC23) UniProt (P38507) Pfam (PF02441) Pfam (PF02216)	Bacillus sp. (strain HIL-Y85/54728)	Mersacidin-modifying enzyme MrsD,IgG-binding protein A,Staphylococcal protein A
2	E, F, G, H (A, B, C, D)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)
3	I, J, K (B, C, D)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 184 (UniProt: Q9RC23)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gln 182	Lys 182	engineered mutation

A, B, C, D: 185 - 231 (UniProt: P38507)

PDB	External Database	Details
Ala 202	Gly 240	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105068.5
Non-Polymers*	Number of molecules	4
	Total formula weight	3142.2
All*	Total formula weight	108210.7

*Water molecules are not included.