5H02

Crystal structure of Methanohalophilus portucalensis glycine sarcosine N-methyltransferase tetramutant (H21G, E23T, E24N, L28S)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycine sarcosine N-methyltransferase	polymer	283	32518.9	1	UniProt (F6KV61) Pfam (PF13649)	Methanohalophilus portucalensis FDF-1
2	B (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	C, D (A)	TRIMETHYL GLYCINE	non-polymer		118.2	2	Chemie (BET)
4	E (A)	water	water		18.0	173	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: F6KV61)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 21	His 21	engineered mutation
Thr 23	Glu 23	engineered mutation
Asn 24	Glu 24	engineered mutation
Ser 28	Leu 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32518.9
Non-Polymers*	Number of molecules	3
	Total formula weight	620.7
All*	Total formula weight	33139.6

*Water molecules are not included.