5GZV

Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Chitinase	polymer	885	98385.7	2	UniProt (K7ZLW6) Pfam (PF00704) Pfam (PF18683)	Paenibacillus sp. FPU-7
2	C, D (C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	E, F (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	G, H (A, B)	water	water		18.0	202	Chemie (HOH)

Sequence modifications

A, B: 557 - 1418 (UniProt: K7ZLW6)

PDB	External Database	Details
Met 534	-	initiating methionine
Asn 535	-	expression tag
His 536	-	expression tag
Lys 537	-	expression tag
Val 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
Ile 545	-	expression tag
Glu 546	-	expression tag
Gly 547	-	expression tag
Arg 548	-	expression tag
His 549	-	expression tag
Met 550	-	expression tag
Glu 551	-	expression tag
Leu 552	-	expression tag
Gly 553	-	expression tag
Thr 554	-	expression tag
Leu 555	-	expression tag
Glu 556	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	196771.3
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	197810.1

*Water molecules are not included.