5GY0
Crystal structure of endoglucanase CelQ from Clostridium thermocellum complexed with cellotetraose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Glucanase | polymer | 610 | 69154.6 | 2 | UniProt (Q9AJF8) Pfam (PF00759) Pfam (PF00942) In PDB | Clostridium thermocellum | |
2 | C, D | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 666.6 | 2 | In PDB BIRD (PRD_900011) GlyTouCan (G00025MO) | beta-cellotetraose | ||
3 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
4 | A, B | CHLORIDE ION | non-polymer | 35.5 | 7 | Chemie (CL) | |||
5 | B | BROMIDE ION | non-polymer | 79.9 | 1 | Chemie (BR) | |||
6 | B | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
7 | water | water | 18.0 | 1406 | Chemie (HOH) |
Sequence modifications
A, B: 28 - 628 (UniProt: Q9AJF8)
PDB | External Database | Details |
---|---|---|
Met 27 | - | initiating methionine |
Thr 251 | Ile 251 | engineered mutation |
Ala 435 | Glu 435 | engineered mutation |
Leu 629 | - | expression tag |
Glu 630 | - | expression tag |
His 631 | - | expression tag |
His 632 | - | expression tag |
His 633 | - | expression tag |
His 634 | - | expression tag |
His 635 | - | expression tag |
His 636 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 138309.1 | |
Branched | Number of molecules | 2 |
Total formula weight | 1333.2 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 682.6 | |
All* | Total formula weight | 140324.9 |