5GTW
The N253R mutant structures of trehalose synthase from Deinococcus radiodurans display two different active-site conformations
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Trehalose synthase | polymer | 571 | 65030.9 | 4 | UniProt (I3NX86) Pfam (PF00128) Pfam (PF16657) In PDB | Deinococcus radiodurans R1 | |
2 | A, B, C, D | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
3 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
4 | A, B, C | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 3 | Chemie (TRS) | |||
5 | water | water | 18.0 | 155 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 552 (UniProt: I3NX86)
PDB | External Database | Details |
---|---|---|
Met -1 | - | expression tag |
Val 0 | - | expression tag |
Pro 1 | - | expression tag |
Trp 97 | Arg 97 | engineered mutation |
Arg 253 | Asn 253 | engineered mutation |
Ile 313 | Thr 313 | engineered mutation |
Val 380 | Ile 380 | engineered mutation |
Ser 553 | - | expression tag |
Arg 554 | - | expression tag |
Val 555 | - | expression tag |
Asp 556 | - | expression tag |
Lys 557 | - | expression tag |
Leu 558 | - | expression tag |
Ala 559 | - | expression tag |
Ala 560 | - | expression tag |
Ala 561 | - | expression tag |
Leu 562 | - | expression tag |
Glu 563 | - | expression tag |
His 564 | - | expression tag |
His 565 | - | expression tag |
His 566 | - | expression tag |
His 567 | - | expression tag |
His 568 | - | expression tag |
His 569 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 260123.8 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 624.0 | |
All* | Total formula weight | 260747.7 |