5GTD

o-Succinylbenzoate CoA Synthetase (MenE) from Bacillus Subtilis in Complex with the Acyl-adenylate Intermediate OSB-AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	2-succinylbenzoate--CoA ligase	polymer	494	55269.1	2	UniProt (P23971) Pfam (PF00501) Pfam (PF13193) In PDB	Bacillus subtilis (strain 168)	o-succinylbenzoyl-CoA synthetase,OSB-CoA synthetase
2	A, B	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	A, B	ADENOSINE-5'-MONOPHOSPHATE	non-polymer		347.2	2	Chemie (A)
5	A, B	2-SUCCINYLBENZOATE	non-polymer		222.2	2	Chemie (OSB)
6	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	B	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
8		water	water		18.0	39	Chemie (HOH)

Sequence modifications

A, B: 1 - 486 (UniProt: P23971)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	110538.2
Non-Polymers*	Number of molecules	11
	Total formula weight	1480.2
All*	Total formula weight	112018.4

*Water molecules are not included.