5GRS

Complex structure of the fission yeast SREBP-SCAP binding domains

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Sterol regulatory element-binding protein cleavage-activating protein	polymer	396	44956.8	4	UniProt (O43043) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	SREBP cleavage-activating protein
2	I, J, K, L	Sterol regulatory element-binding protein cleavage-activating protein	polymer	103	11868.6	4	UniProt (O43043) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	SREBP cleavage-activating protein
3	E, F, G, H	Sterol regulatory element-binding protein 1	polymer	272	30318.8	4	UniProt (Q9UUD1) Pfam (PF09427) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Sequence modifications

A, B, C, D: 567 - 961 (UniProt: O43043)

PDB	External Database	Details
Met 566	-	expression tag
Ser 671	Cys 671	engineered mutation
Ser 873	Cys 873	engineered mutation
Ser 901	Cys 901	engineered mutation
Ser 920	Cys 920	engineered mutation
Ser 941	Cys 941	engineered mutation

I, J, K, L: 986 - 1085 (UniProt: O43043)

PDB	External Database	Details
Ala 983	-	expression tag
His 984	-	expression tag
Met 985	-	expression tag
Ser 1010	Cys 1010	engineered mutation

E, F, G, H: 628 - 896 (UniProt: Q9UUD1)

PDB	External Database	Details
Ala 625	-	expression tag
His 626	-	expression tag
Met 627	-	expression tag
Ser 644	Cys 644	engineered mutation
Ser 672	Cys 672	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	348576.7
All*	Total formula weight	348576.7

*Water molecules are not included.