5GR4
Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 1,4-alpha-glucan branching enzyme GlgB | polymer | 793 | 92644.3 | 1 | UniProt (B1WPM8) Pfam (PF22019) Pfam (PF02922) Pfam (PF00128) Pfam (PF02806) | Cyanothece sp. (strain ATCC 51142) | 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase,Alpha-(1->4)-glucan branching enzyme,Glycogen branching enzyme |
| 2 | B (B) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB BIRD (PRD_900009) GlyTouCan (G96370VA) | alpha-maltotriose | ||
| 3 | C (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | D, F (D, F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1153.0 | 2 | In PDB GlyTouCan (G41288IQ) | |||
| 5 | E, G (E, G) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 2 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | alpha-maltotetraose | ||
| 6 | H, I (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 7 | J, K, L, M (A) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 8 | N (A) | water | water | 18.0 | 611 | Chemie (HOH) |
Sequence modifications
A: 1 - 773 (UniProt: B1WPM8)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 541 | Leu 541 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 92644.3 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 4485.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 281.4 | |
| All* | Total formula weight | 97411.6 |
