5GR3

Crystal structure of branching enzyme L541A/W655A mutant from Cyanothece sp. ATCC 51142

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1,4-alpha-glucan branching enzyme GlgB	polymer	793	92529.2	1	UniProt (B1WPM8) Pfam (PF22019) Pfam (PF02922) Pfam (PF00128) Pfam (PF02806) In PDB	Cyanothece sp. (strain ATCC 51142)	1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase,Alpha-(1->4)-glucan branching enzyme,Glycogen branching enzyme
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4		water	water		18.0	477	Chemie (HOH)

Sequence modifications

A: 1 - 773 (UniProt: B1WPM8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 541	Leu 541	engineered mutation
Ala 655	Trp 655	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	92529.2
Non-Polymers*	Number of molecules	3
	Total formula weight	140.7
All*	Total formula weight	92669.9

*Water molecules are not included.