5GQZ

Crystal structure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1,4-alpha-glucan branching enzyme GlgB	polymer	793	92594.3	1	UniProt (B1WPM8) Pfam (PF22019) Pfam (PF02922) Pfam (PF00128) Pfam (PF02806) In PDB	Cyanothece sp. (strain ATCC 51142)	1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase,Alpha-(1->4)-glucan branching enzyme,Glycogen branching enzyme
2	A	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
3	A	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4		water	water		18.0	889	Chemie (HOH)

Sequence modifications

A: 1 - 773 (UniProt: B1WPM8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 500	Tyr 500	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	92594.3
Non-Polymers*	Number of molecules	13
	Total formula weight	926.1
All*	Total formula weight	93520.4

*Water molecules are not included.