5GQE
Crystal structure of michaelis complex of xylanase mutant (T82A, N127S, and E128H) from Streptomyces olivaceoviridis E-86
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-xylanase | polymer | 436 | 46743.2 | 2 | UniProt (Q7SI98) Pfam (PF00331) Pfam (PF00652) | Streptomyces olivaceoviridis | |
| 2 | C, F (C, F) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 678.6 | 2 | In PDB GlyTouCan (G47101GE) | |||
| 3 | D (D) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 546.5 | 1 | In PDB GlyTouCan (G60650FG) | |||
| 4 | E, G (E, G) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 282.2 | 2 | In PDB BIRD (PRD_900116) GlyTouCan (G87728WL) | 4beta-beta-xylobiose | ||
| 5 | H (H) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 414.4 | 1 | In PDB BIRD (PRD_900117) GlyTouCan (G87429QT) | 4beta-beta-xylotriose | ||
| 6 | I, J (A, B) | water | water | 18.0 | 727 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 436 (UniProt: Q7SI98)
| PDB | External Database | Details |
|---|---|---|
| Ala 82 | Thr 82 | engineered mutation |
| Ser 127 | Asn 127 | engineered mutation |
| His 128 | Glu 128 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 93486.4 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 2882.5 | |
| All* | Total formula weight | 96368.9 |
