5GO6

Structure of sortase E T196V mutant from Streptomyces avermitilis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative secreted protein	polymer	201	22326.3	1	UniProt (Q82FC3) Pfam (PF04203)	Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)	Sortase E
2	B, C (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D (A)	water	water		18.0	98	Chemie (HOH)

Sequence modifications

A: 51 - 230 (UniProt: Q82FC3)

PDB	External Database	Details
Met 30	-	expression tag
Gly 31	-	expression tag
Ser 32	-	expression tag
Ser 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
Ser 40	-	expression tag
Ser 41	-	expression tag
Gly 42	-	expression tag
Leu 43	-	expression tag
Val 44	-	expression tag
Pro 45	-	expression tag
Arg 46	-	expression tag
Gly 47	-	expression tag
Ser 48	-	expression tag
His 49	-	expression tag
Met 50	-	expression tag
Val 196	Thr 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22326.3
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	22518.4

*Water molecules are not included.