5GMR

Crystal structure of the mutant M3+S202W/I203F of the esterase E40

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (C, A, B, D)	Esterase	polymer	315	34314.1	4	UniProt (A0A0F6WGE1) Pfam (PF07859)	uncultured bacterium	Esterase E40
2	E, F, G, H (C, A, B, D)	water	water		18.0	1325	Chemie (HOH)

Sequence modifications

C, A, B, D: 1 - 299 (UniProt: A0A0F6WGE1)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Tyr 22	Arg 22	see sequence details
His 24	-	see sequence details
Leu 25	-	see sequence details
Ser 26	Thr 24	see sequence details
Trp 204	Ser 202	engineered mutation
Phe 205	Ile 203	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	137256.5
All*	Total formula weight	137256.5

*Water molecules are not included.