5GHZ

Crystal structure of beta-lactamase PenP mutant-E166H in complex with cephaloridine as "pre-deacylation" intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Beta-lactamase	polymer	268	29836.8	2	UniProt (P00808) Pfam (PF00144) In PDB	Bacillus licheniformis	Penicillinase
2	B, A	5-METHYL-2-[2-OXO-1-(2-THIOPHEN-2-YL-ACETYLAMINO)-ETHYL]-3,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC ACID	non-polymer		340.4	2	Chemie (CED)
3		water	water		18.0	484	Chemie (HOH)

Sequence modifications

B, A: 26 - 295 (UniProt: P00808)

PDB	External Database	Details
Gly 23	-	expression tag
Pro 24	-	expression tag
Met 25	-	expression tag
His 166	Glu 180	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59673.7
Non-Polymers*	Number of molecules	2
	Total formula weight	680.8
All*	Total formula weight	60354.5

*Water molecules are not included.