5GGI

Crystal structure of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with Mn, UDP and Mannosyl-peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1	polymer	578	65551.5	2	UniProt (Q8WZA1) Pfam (PF15711) Pfam (PF03071)	Homo sapiens (Human)	POMGnT1,UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2,GnT I.2
2	C, D (F, G)	mannosyl-peptide	polymer	11	818.0	2		Homo sapiens
3	E, H (A, B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
4	F, I (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	G (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	J, K (F, G)	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
7	L, M, N (A, B, G)	water	water		18.0	101	Chemie (HOH)

Sequence modifications

A, B: 92 - 660 (UniProt: Q8WZA1)

PDB	External Database	Details
Val 623	Met 623	variant
Leu 661	-	expression tag
Glu 662	-	expression tag
Leu 663	-	expression tag
Glu 664	-	expression tag
Val 665	-	expression tag
Leu 666	-	expression tag
Phe 667	-	expression tag
Gln 668	-	expression tag
Gly 669	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132738.8
Non-Polymers*	Number of molecules	7
	Total formula weight	1373.5
All*	Total formula weight	134112.3

*Water molecules are not included.