5G1K

A triple mutant of DsbG engineered for denitrosylation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	THIOL DISULFIDE INTERCHANGE PROTEIN DSBG	polymer	256	28536.1	2	UniProt (P77202) Pfam (PF10411) Pfam (PF13098) In PDB	ESCHERICHIA COLI
2	A, B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A, B: -16 - 231 (UniProt: P77202)

PDB	External Database	Details
Thr 232	-	expression tag
Arg 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
Gly 110	Pro 127	engineered mutation
Pro 111	Tyr 128	engineered mutation
Met 200	Thr 217	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57072.2
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	57360.4

*Water molecules are not included.