5FU6

NOT module of the human CCR4-NOT complex (Crystallization mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1	polymer	535	61620.1	2	UniProt (A5YKK6) Pfam (PF04054)	HOMO SAPIENS (HUMAN)	CCR4-ASSOCIATED FACTOR 1, NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1 HOMOLOG, NOT1H, HNOT1, CNOT1
2	B, E (B, E)	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 2	polymer	197	22945.1	2	UniProt (Q9NZN8) Pfam (PF04153)	HOMO SAPIENS (HUMAN)	CCR4-ASSOCIATED FACTOR 2, CNOT2
3	C, F (C, F)	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 3	polymer	148	18267.6	2	UniProt (O75175) Pfam (PF04153)	HOMO SAPIENS (HUMAN)	CCR4-ASSOCIATED FACTOR 3, LEUKOCYTE RECEPTOR CLUSTER MEMBER 2, CNOT3

Sequence modifications

A, D: 1833 - 2361 (UniProt: A5YKK6)

PDB	External Database	Details
Gly 1827	-	expression tag
Pro 1828	-	expression tag
His 1829	-	expression tag
Met 1830	-	expression tag
Leu 1831	-	expression tag
Glu 1832	-	expression tag
Glu 2344	His 2344	engineered mutation
Glu 2345	Cys 2345	engineered mutation
Glu 2346	Ala 2346	engineered mutation

B, E: 350 - 540 (UniProt: Q9NZN8)

PDB	External Database	Details
Gly 344	-	expression tag
Pro 345	-	expression tag
His 346	-	expression tag
Met 347	-	expression tag
Leu 348	-	expression tag
Glu 349	-	expression tag

C, F: 607 - 748 (UniProt: O75175)

PDB	External Database	Details
Gly 601	-	expression tag
Pro 602	-	expression tag
His 603	-	expression tag
Met 604	-	expression tag
Leu 605	-	expression tag
Glu 606	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205665.5
All*	Total formula weight	205665.5

*Water molecules are not included.