5FQH

The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	BETA-N-ACETYLGALACTOSAMINIDASE	polymer	610	70232.3	1	UniProt (A0A0H2YNR7) Pfam (PF22680) Pfam (PF13320)	CLOSTRIDIUM PERFRINGENS
2	B (B)	2-acetamido-2-deoxy-beta-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose	branched		545.5	1	In PDB GlyTouCan (G90562PB)
3	C (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	D (A)	water	water		18.0	166	Chemie (HOH)

Sequence modifications

A: 1 - 587 (UniProt: A0A0H2YNR7)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Asn 344	Asp 344	engineered mutation
Gln 345	Glu 345	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	70232.3
Branched	Number of molecules	1
	Total formula weight	545.5
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	70872.7

*Water molecules are not included.