5FLK

Structure of haloalkane dehalogenase variant DhaA101

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DHAA101	polymer	300	34234.0	1	UniProt (P0A3G2) Pfam (PF00561)	RHODOCOCCUS RHODOCHROUS
2	B (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
3	C, D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	E (A)	water	water		18.0	316	Chemie (HOH)

Sequence modifications

A: 1 - 293 (UniProt: P0A3G2)

PDB	External Database	Details
Glu 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
Ser 20	Glu 20	engineered mutation
Arg 80	Phe 80	engineered mutation
Pro 155	Ala 155	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34234.0
Non-Polymers*	Number of molecules	3
	Total formula weight	407.5
All*	Total formula weight	34641.5

*Water molecules are not included.