5FKC

Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with Raffinose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	BETA-FRUCTOFURANOSIDASE	polymer	663	71776.8	2	UniProt (J7HDY4) Pfam (PF00251) Pfam (PF08244) In PDB	Xanthophyllomyces dendrorhous
2	C, G	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
3	D, H	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	2	In PDB GlyTouCan (G40702WU)
4	E, I	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	F, J	alpha-D-galactopyranose-(1-6)-alpha-D-glucopyranose-(1-2)-beta-D-fructofuranose	branched		504.4	2	In PDB BIRD (PRD_900002)		raffinose
6	B, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	27	Chemie (NAG)
7		water	water		18.0	1597	Chemie (HOH)

Sequence modifications

A, B: 3 - 665 (UniProt: J7HDY4)

PDB	External Database	Details
Ala 663	Ser 663	conflict
Tyr 665	Arg 665	conflict
Ala 80	Asp 80	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143553.7
Branched	Number of molecules	8
	Total formula weight	7446.7
Non-Polymers*	Number of molecules	27
	Total formula weight	5972.6
All*	Total formula weight	156972.9

*Water molecules are not included.