5FHT

HtrA2 protease mutant V226K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Serine protease HTRA2, mitochondrial	polymer	334	36234.2	1	UniProt (O43464) Pfam (PF13365) Pfam (PF17820) In PDB	Homo sapiens (Human)	High temperature requirement protein A2,HtrA2,Omi stress-regulated endoprotease,Serine protease 25,Serine proteinase OMI
2	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6		water	water		18.0	200	Chemie (HOH)

Sequence modifications

A: 1 - 325 (UniProt: O43464)

PDB	External Database	Details
Met 0	-	initiating methionine
Lys 93	Val 226	engineered mutation
Ala 173	Ser 306	engineered mutation
Leu 326	-	expression tag
Glu 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
His 333	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36234.2
Non-Polymers*	Number of molecules	4
	Total formula weight	292.8
All*	Total formula weight	36527.0

*Water molecules are not included.