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5FBG

S1 nuclease from Aspergillus oryzae, mutant D65N, in complex with phosphate, 2'-deoxycytidine and 2'-deoxyguanosine.

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B
(A, B)
Nuclease S1polymer26729082.72UniProt (P24021)
Pfam (PF02265)
Aspergillus oryzae RIB40 (Yellow koji mold)Deoxyribonuclease S1,Endonuclease S1,Single-stranded-nucleate endonuclease
2C, D, E, K, L...
(A, B)
ZINC IONnon-polymer65.46Chemie (ZN)
3F, G, N, O
(A, B)
2-acetamido-2-deoxy-beta-D-glucopyranosenon-polymer221.24Chemie (NAG)
4H, P
(A, B)
PHOSPHATE IONnon-polymer95.02Chemie (PO4)
5I, J
(A)
2'-DEOXYCYTIDINEnon-polymer227.22Chemie (DCZ)
6Q
(B)
2'-DEOXY-GUANOSINEnon-polymer267.21Chemie (GNG)
7R, S
(A, B)
waterwater18.0652Chemie (HOH)
Sequence modifications
A, B: 21 - 287 (UniProt: P24021)
PDBExternal DatabaseDetails
Asn 65Asp 65engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains2
Total formula weight58165.4
Non-Polymers*Number of molecules15
Total formula weight2188.9
All*Total formula weight60354.3
*Water molecules are not included.

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PDB entries from 2025-12-24

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