5F7Y

Blood group antigen binding adhesin BabA of Helicobacter pylori strain 17875 in complex with blood group A type-1 hexasaccharide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adhesin binding fucosylated histo-blood group antigen	polymer	466	49397.3	1	UniProt (O52269) Pfam (PF18304)	Helicobacter pylori	Blood group antigen binding adhesin (BabA), strain 17875
2	B (B)	Adhesin binding fucosylated histo-blood group antigen	polymer	466	49369.2	1	UniProt (O52269) Pfam (PF18304)	Helicobacter pylori
3	C, D (C, D)	Nanobody Nb-ER19	polymer	120	13260.9	2		Lama glama (Llama)
4	E, F (E, F)	alpha-L-fucopyranose-(1-2)-[2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)]beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose	branched		1057.0	2	In PDB GlyTouCan (G56336GK)
5	G, H, I, J (A, B, C, D)	water	water		18.0	137	Chemie (HOH)

Sequence modifications

A: 25 - 460 (UniProt: O52269)

PDB	External Database	Details
Ala 3	-	expression tag
Ser 4	-	expression tag
Trp 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
Pro 8	-	expression tag
Gln 9	-	expression tag
Phe 10	-	expression tag
Glu 11	-	expression tag
Lys 12	-	expression tag
Ser 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Gly 16	-	expression tag
Gly 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Val 20	-	expression tag
Pro 21	-	expression tag
Arg 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
Lys 226	Thr 246	conflict
Gly 461	-	expression tag
Ser 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag

B: 25 - 460 (UniProt: O52269)

PDB	External Database	Details
Ala 3	-	expression tag
Ser 4	-	expression tag
Trp 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
Pro 8	-	expression tag
Gln 9	-	expression tag
Phe 10	-	expression tag
Glu 11	-	expression tag
Lys 12	-	expression tag
Ser 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Gly 16	-	expression tag
Gly 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Val 20	-	expression tag
Pro 21	-	expression tag
Arg 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
Gly 461	-	expression tag
Ser 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	125288.2
Branched	Number of molecules	2
	Total formula weight	2113.9
All*	Total formula weight	127402.1

*Water molecules are not included.