5F77

Crystal structure of Mutant S12T of adenosine/Methylthioadenosine phosphorylase from Schistosoma mansoni in complex with Adenine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Methylthioadenosine phosphorylase	polymer	320	35273.5	3	UniProt (I0B503) Pfam (PF01048)	Schistosoma mansoni (Blood fluke)
2	D, F, H (A, B, C)	ADENINE	non-polymer		135.1	3	Chemie (ADE)
3	E, G, I (A, B, C)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	J, K, L (A, B, C)	water	water		18.0	647	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 299 (UniProt: I0B503)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Thr 12	Ser 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	105820.5
Non-Polymers*	Number of molecules	6
	Total formula weight	693.6
All*	Total formula weight	106514.1

*Water molecules are not included.