5EX6

Structure of P450 StaH from glycopeptide antibiotic A47934 biosynthesis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (C, A, B)	Cytochrome P450	polymer	434	47965.4	3	UniProt (Q8KLL9) Pfam (PF00067)	Streptomyces toyocaensis	StaH
2	D, G, H (C, A)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
3	E, I, L (C, A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	3	Chemie (HEM)	Streptomyces toyocaensis	StaH
4	F, J, K (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	M, N, O (C, A, B)	water	water		18.0	233	Chemie (HOH)

Sequence modifications

C, A, B: 1 - 398 (UniProt: Q8KLL9)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	143896.2
Non-Polymers*	Number of molecules	9
	Total formula weight	2311.9
All*	Total formula weight	146208.2

*Water molecules are not included.