5ESF

Saccharomyces cerevisiae CYP51 (Lanosterol 14-alpha demethylase) G73E mutant complexed with fluconazole

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lanosterol 14-alpha demethylase	polymer	539	61957.3	1	UniProt (A6ZSR0) Pfam (PF00067) In PDB	Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL	non-polymer		306.3	1	Chemie (TPF)
4		water	water		18.0	107	Chemie (HOH)

Sequence modifications

A: 1 - 530 (UniProt: A6ZSR0)

PDB	External Database	Details
Glu 73	Gly 73	engineered mutation
Gly 531	-	expression tag
Gly 532	-	expression tag
Arg 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	61957.3
Non-Polymers*	Number of molecules	2
	Total formula weight	922.8
All*	Total formula weight	62880.0

*Water molecules are not included.