5ERL

Crystal structure of the epimerase SnoN in complex with Ni2+, succinate and nogalamycin RO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	SnoN,SnoN	polymer	305	33079.0	4	UniProt (Q9RN67) UniProt (Q9EYI0) Pfam (PF05721)	Streptomyces nogalater
2	E, H, K, N (A, B, C, D)	NICKEL (II) ION	non-polymer		58.7	4	Chemie (NI)
3	F, I, L, O (A, B, C, D)	SUCCINIC ACID	non-polymer		118.1	4	Chemie (SIN)
4	G, J, M, P (A, B, C, D)	Nogalamycin RO	non-polymer		699.7	4	Chemie (5R6)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	15	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 190 (UniProt: Q9RN67)

PDB	External Database	Details
Met -11	-	initiating methionine
Ala -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Arg -2	-	expression tag
Ser -1	-	expression tag
Ala 0	-	expression tag
Asp 1	-	expression tag

A, B, C, D: 191 - 293 (UniProt: Q9EYI0)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132316.1
Non-Polymers*	Number of molecules	12
	Total formula weight	3505.9
All*	Total formula weight	135822.0

*Water molecules are not included.