5EL4

Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, DB (13, 1G)	16S ribosomal RNA	polymer	1522	493997.3	2	GenBank (55771382)	Thermus thermophilus HB8
2	B, EB (1E, 12)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus HB8
3	C, FB (2E, 22)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus HB8
4	D, GB (3E, 32)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus HB8
5	E, HB (4E, 42)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus HB8
6	F, IB (5E, 52)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus HB8	TS9
7	G, JB (6E, 62)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus HB8
8	H, KB (7E, 72)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2)	Thermus thermophilus HB8
9	I, LB (8E, 82)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus HB8
10	J, MB (1I, 1A)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus HB8
11	K, NB (2I, 2A)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus HB8
12	L, OB (3I, 3A)	30S ribosomal protein S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus HB8
13	M, PB (4I, 4A)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus HB8
14	N, QB (5I, 5A)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1)	Thermus thermophilus HB8
15	O, RB (6I, 6A)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus HB8
16	P, SB (7I, 7A)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus HB8
17	Q, TB (8I, 8A)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7)	Thermus thermophilus HB8
18	R, UB (9I, 9A)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus HB8
19	S, VB (AI, AA)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus HB8
20	T, WB (BI, BA)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus HB8
21	U, XB (1F, 1B)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus HB8	S31
22	V (1K)	tRNA-Lys	polymer	76	24612.7	1	GenBank (937517453)	Escherichia coli K-12
23	W, ZB (2K, 2L)	tRNA-fMet	polymer	77	24862.9	2		Escherichia coli K-12
24	X (3K)	tRNA-Lys	polymer	76	24378.4	1	GenBank (937517453)	Escherichia coli K-12
25	BC, Y (4K, 4L)	mRNA	polymer	27	8862.5	2		Escherichia coli
26	CC, Z (1H, 14)	23S ribosomal RNA	polymer	2917	948539.9	2	GenBank (55771382)	Thermus thermophilus HB8
27	AA, DC (16, 1J)	5S ribosomal RNA	polymer	122	39540.6	2	GenBank (55771382)	Thermus thermophilus HB8
28	BA (71)	50S ribosomal protein L1	polymer	229	24867.7	1	UniProt (Q5SLP7) Pfam (PF00687)	Thermus thermophilus HB8
29	CA, EC (11, 19)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus HB8
30	DA, FC (21, 29)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus HB8
31	EA, GC (31, 39)	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus HB8	L1e
32	FA, HC (41, 49)	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus HB8
33	GA, IC (51, 59)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3)	Thermus thermophilus HB8
34	HA, JC (61, 69)	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus HB8
35	IA, KC (58, 15)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus HB8
36	JA, LC (68, 25)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus HB8
37	KA, MC (78, 35)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus HB8
38	LA, NC (88, 45)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus HB8
39	MA, OC (98, 55)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus HB8
40	NA, PC (A8, 65)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus HB8	TL24
41	OA, QC (B8, 75)	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus HB8
42	PA, RC (C8, 85)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus HB8
43	QA, SC (D8, 95)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus HB8
44	RA, TC (E8, A5)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus HB8
45	SA, UC (F8, B5)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus HB8
46	TA, VC (G8, C5)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus HB8
47	UA, WC (H8, D5)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus HB8	TL5
48	VA, XC (I8, E5)	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus HB8
49	WA, YC (J8, F5)	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus HB8
50	XA, ZC (K8, G5)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus HB8
51	AD, YA (L8, H5)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus HB8
52	ZA (M8)	50S ribosomal protein L31	polymer	71	8300.5	1	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus HB8
53	AB, BD (N8, J5)	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus HB8
54	BB, CD (P8, L5)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus HB8
55	CB, DD (Q8, M5)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus HB8
56	YB (1L)	tRNA-Lys	polymer	76	24392.4	1	GenBank (937517453)	Escherichia coli K-12
57	AC (3L)	tRNA-Lys	polymer	76	24378.4	1	GenBank (937517453)	Escherichia coli K-12
58	AAA, ABA, ACA, ADA, AE... (1H, P8, 13, 1G, 14...)	MAGNESIUM ION	non-polymer		24.3	1207	Chemie (MG)
59	QI, TGA (3E, 32)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
60	TXA, UGA, WCA, WI (C5, 5A, G8, 5I)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
61	AYA, AZA, BYA, BZA, CYA... (3I, 39, 5I, 15, 6I...)	water	water		18.0	1973	Chemie (HOH)

Sequence modifications

13, 1G: 0 - 1541 (GenBank: 55771382)

PDB	External Database	Details
G 1542	-	insertion
C 1543	-	insertion
U 1544	-	insertion

1H, 14: 1 - 2907 (GenBank: 55771382)

PDB	External Database	Details
U 161	Unk 9395	conflict
A 654	G 9920	conflict
C 654	G 9924	conflict
G 654	C 9935	conflict
A 654	C 9939	conflict
U 1058	G 10344	conflict
A 1080	C 10366	conflict

71: 0 - 228 (UniProt: Q5SLP7)

PDB	External Database	Details
Ile 19	Val 20	conflict
His 27	Arg 28	conflict

Contents of the asymmetric unit

Polymers	Number of chains	108
	Total formula weight	4498518.4
Non-Polymers*	Number of molecules	1213
	Total formula weight	30301.1
All*	Total formula weight	4528819.4

*Water molecules are not included.