5EL4
Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | 13, 1G | 16S ribosomal RNA | polymer | 1522 | 493997.3 | 2 | GenBank (55771382) | Thermus thermophilus HB8 | |
2 | 1E, 12 | 30S ribosomal protein S2 | polymer | 256 | 29317.7 | 2 | UniProt (P80371) Pfam (PF00318) In PDB | Thermus thermophilus HB8 | |
3 | 2E, 22 | 30S ribosomal protein S3 | polymer | 239 | 26751.1 | 2 | UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB | Thermus thermophilus HB8 | |
4 | 3E, 32 | 30S ribosomal protein S4 | polymer | 209 | 24373.4 | 2 | UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB | Thermus thermophilus HB8 | |
5 | 4E, 42 | 30S ribosomal protein S5 | polymer | 162 | 17583.4 | 2 | UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB | Thermus thermophilus HB8 | |
6 | 5E, 52 | 30S ribosomal protein S6 | polymer | 101 | 11988.8 | 2 | UniProt (Q5SLP8) Pfam (PF01250) In PDB | Thermus thermophilus HB8 | TS9 |
7 | 6E, 62 | 30S ribosomal protein S7 | polymer | 156 | 18051.0 | 2 | UniProt (P17291) Pfam (PF00177) In PDB | Thermus thermophilus HB8 | |
8 | 7E, 72 | 30S ribosomal protein S8 | polymer | 138 | 15868.6 | 2 | UniProt (Q5SHQ2) In PDB | Thermus thermophilus HB8 | |
9 | 8E, 82 | 30S ribosomal protein S9 | polymer | 128 | 14410.6 | 2 | UniProt (P80374) Pfam (PF00380) In PDB | Thermus thermophilus HB8 | |
10 | 1I, 1A | 30S ribosomal protein S10 | polymer | 105 | 11955.0 | 2 | UniProt (Q5SHN7) Pfam (PF00338) In PDB | Thermus thermophilus HB8 | |
11 | 2I, 2A | 30S ribosomal protein S11 | polymer | 129 | 13737.9 | 2 | UniProt (P80376) Pfam (PF00411) In PDB | Thermus thermophilus HB8 | |
12 | 3I, 3A | 30S ribosomal protein S12 | polymer | 132 | 14637.4 | 2 | UniProt (Q5SHN3) Pfam (PF00164) In PDB | Thermus thermophilus HB8 | |
13 | 4I, 4A | 30S ribosomal protein S13 | polymer | 126 | 14338.9 | 2 | UniProt (P80377) Pfam (PF00416) In PDB | Thermus thermophilus HB8 | |
14 | 5I, 5A | 30S ribosomal protein S14 type Z | polymer | 61 | 7158.7 | 2 | UniProt (Q5SHQ1) In PDB | Thermus thermophilus HB8 | |
15 | 6I, 6A | 30S ribosomal protein S15 | polymer | 89 | 10578.4 | 2 | UniProt (Q5SJ76) Pfam (PF00312) In PDB | Thermus thermophilus HB8 | |
16 | 7I, 7A | 30S ribosomal protein S16 | polymer | 88 | 10410.0 | 2 | UniProt (Q5SJH3) Pfam (PF00886) In PDB | Thermus thermophilus HB8 | |
17 | 8I, 8A | 30S ribosomal protein S17 | polymer | 105 | 12325.7 | 2 | UniProt (Q5SHP7) In PDB | Thermus thermophilus HB8 | |
18 | 9I, 9A | 30S ribosomal protein S18 | polymer | 88 | 10258.3 | 2 | UniProt (Q5SLQ0) Pfam (PF01084) In PDB | Thermus thermophilus HB8 | |
19 | AI, AA | 30S ribosomal protein S19 | polymer | 93 | 10605.5 | 2 | UniProt (Q5SHP2) Pfam (PF00203) In PDB | Thermus thermophilus HB8 | |
20 | BI, BA | 30S ribosomal protein S20 | polymer | 106 | 11736.1 | 2 | UniProt (P80380) Pfam (PF01649) In PDB | Thermus thermophilus HB8 | |
21 | 1F, 1B | 30S ribosomal protein Thx | polymer | 27 | 3350.0 | 2 | UniProt (Q5SIH3) Pfam (PF17070) In PDB | Thermus thermophilus HB8 | S31 |
22 | 1K | tRNA-Lys | polymer | 76 | 24612.7 | 1 | GenBank (937517453) | Escherichia coli K-12 | |
23 | 2K, 2L | tRNA-fMet | polymer | 77 | 24862.9 | 2 | Escherichia coli K-12 | ||
24 | 3K | tRNA-Lys | polymer | 76 | 24378.4 | 1 | GenBank (937517453) | Escherichia coli K-12 | |
25 | 4K, 4L | mRNA | polymer | 27 | 8862.5 | 2 | Escherichia coli | ||
26 | 1H, 14 | 23S ribosomal RNA | polymer | 2917 | 948539.9 | 2 | GenBank (55771382) | Thermus thermophilus HB8 | |
27 | 16, 1J | 5S ribosomal RNA | polymer | 122 | 39540.6 | 2 | GenBank (55771382) | Thermus thermophilus HB8 | |
28 | 71 | 50S ribosomal protein L1 | polymer | 229 | 24867.7 | 1 | UniProt (Q5SLP7) Pfam (PF00687) In PDB | Thermus thermophilus HB8 | |
29 | 11, 19 | 50S ribosomal protein L2 | polymer | 276 | 30532.6 | 2 | UniProt (P60405) Pfam (PF00181) Pfam (PF03947) In PDB | Thermus thermophilus HB8 | |
30 | 21, 29 | 50S ribosomal protein L3 | polymer | 206 | 22450.2 | 2 | UniProt (Q5SHN8) Pfam (PF00297) In PDB | Thermus thermophilus HB8 | |
31 | 31, 39 | 50S ribosomal protein L4 | polymer | 210 | 23271.9 | 2 | UniProt (Q5SHN9) Pfam (PF00573) In PDB | Thermus thermophilus HB8 | L1e |
32 | 41, 49 | 50S ribosomal protein L5 | polymer | 182 | 21061.6 | 2 | UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673) In PDB | Thermus thermophilus HB8 | |
33 | 51, 59 | 50S ribosomal protein L6 | polymer | 180 | 19568.9 | 2 | UniProt (Q5SHQ3) In PDB | Thermus thermophilus HB8 | |
34 | 61, 69 | 50S ribosomal protein L9 | polymer | 148 | 16422.2 | 2 | UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948) In PDB | Thermus thermophilus HB8 | |
35 | 58, 15 | 50S ribosomal protein L13 | polymer | 140 | 15927.9 | 2 | UniProt (P60488) Pfam (PF00572) In PDB | Thermus thermophilus HB8 | |
36 | 68, 25 | 50S ribosomal protein L14 | polymer | 122 | 13323.6 | 2 | UniProt (Q5SHP8) Pfam (PF00238) In PDB | Thermus thermophilus HB8 | |
37 | 78, 35 | 50S ribosomal protein L15 | polymer | 150 | 16319.1 | 2 | UniProt (Q5SHQ7) Pfam (PF00828) In PDB | Thermus thermophilus HB8 | |
38 | 88, 45 | 50S ribosomal protein L16 | polymer | 141 | 15993.9 | 2 | UniProt (P60489) Pfam (PF00252) In PDB | Thermus thermophilus HB8 | |
39 | 98, 55 | 50S ribosomal protein L17 | polymer | 118 | 13750.1 | 2 | UniProt (Q9Z9H5) Pfam (PF01196) In PDB | Thermus thermophilus HB8 | |
40 | A8, 65 | 50S ribosomal protein L18 | polymer | 112 | 12639.8 | 2 | UniProt (Q5SHQ4) Pfam (PF00861) In PDB | Thermus thermophilus HB8 | TL24 |
41 | B8, 75 | 50S ribosomal protein L19 | polymer | 146 | 17188.8 | 2 | UniProt (P60490) Pfam (PF01245) In PDB | Thermus thermophilus HB8 | |
42 | C8, 85 | 50S ribosomal protein L20 | polymer | 118 | 13779.3 | 2 | UniProt (P60491) Pfam (PF00453) In PDB | Thermus thermophilus HB8 | |
43 | D8, 95 | 50S ribosomal protein L21 | polymer | 101 | 11069.2 | 2 | UniProt (P60492) Pfam (PF00829) In PDB | Thermus thermophilus HB8 | |
44 | E8, A5 | 50S ribosomal protein L22 | polymer | 113 | 12808.1 | 2 | UniProt (Q5SHP3) Pfam (PF00237) In PDB | Thermus thermophilus HB8 | |
45 | F8, B5 | 50S ribosomal protein L23 | polymer | 96 | 10759.8 | 2 | UniProt (Q5SHP0) Pfam (PF00276) In PDB | Thermus thermophilus HB8 | |
46 | G8, C5 | 50S ribosomal protein L24 | polymer | 110 | 12085.6 | 2 | UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136) In PDB | Thermus thermophilus HB8 | |
47 | H8, D5 | 50S ribosomal protein L25 | polymer | 206 | 23238.6 | 2 | UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693) In PDB | Thermus thermophilus HB8 | TL5 |
48 | I8, E5 | 50S ribosomal protein L27 | polymer | 85 | 9529.1 | 2 | UniProt (P60493) Pfam (PF01016) In PDB | Thermus thermophilus HB8 | |
49 | J8, F5 | 50S ribosomal protein L28 | polymer | 98 | 11004.2 | 2 | UniProt (P60494) Pfam (PF00830) In PDB | Thermus thermophilus HB8 | |
50 | K8, G5 | 50S ribosomal protein L29 | polymer | 72 | 8670.3 | 2 | UniProt (Q5SHP6) Pfam (PF00831) In PDB | Thermus thermophilus HB8 | |
51 | L8, H5 | 50S ribosomal protein L30 | polymer | 60 | 6799.1 | 2 | UniProt (Q5SHQ6) Pfam (PF00327) In PDB | Thermus thermophilus HB8 | |
52 | M8 | 50S ribosomal protein L31 | polymer | 71 | 8300.5 | 1 | UniProt (Q5SJE1) Pfam (PF01197) In PDB | Thermus thermophilus HB8 | |
53 | N8, J5 | 50S ribosomal protein L32 | polymer | 60 | 6722.1 | 2 | UniProt (P80339) Pfam (PF01783) In PDB | Thermus thermophilus HB8 | |
54 | P8, L5 | 50S ribosomal protein L34 | polymer | 49 | 6132.4 | 2 | UniProt (P80340) Pfam (PF00468) In PDB | Thermus thermophilus HB8 | |
55 | Q8, M5 | 50S ribosomal protein L35 | polymer | 65 | 7506.2 | 2 | UniProt (Q5SKU1) Pfam (PF01632) In PDB | Thermus thermophilus HB8 | |
56 | 1L | tRNA-Lys | polymer | 76 | 24392.4 | 1 | GenBank (937517453) | Escherichia coli K-12 | |
57 | 3L | tRNA-Lys | polymer | 76 | 24378.4 | 1 | GenBank (937517453) | Escherichia coli K-12 | |
58 | 1H, P8, 13, 1G, 14... | MAGNESIUM ION | non-polymer | 24.3 | 1207 | Chemie (MG) | |||
59 | 3E, 32 | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
60 | C5, 5A, G8, 5I | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
61 | water | water | 18.0 | 1973 | Chemie (HOH) |
Sequence modifications
13, 1G: 0 - 1541 (GenBank: 55771382)
1H, 14: 1 - 2907 (GenBank: 55771382)
71: 0 - 228 (UniProt: Q5SLP7)
PDB | External Database | Details |
---|---|---|
G 1542 | - | insertion |
C 1543 | - | insertion |
U 1544 | - | insertion |
PDB | External Database | Details |
---|---|---|
U 161 | Unk 9395 | conflict |
A 654 | G 9920 | conflict |
C 654 | G 9924 | conflict |
G 654 | C 9935 | conflict |
A 654 | C 9939 | conflict |
U 1058 | G 10344 | conflict |
A 1080 | C 10366 | conflict |
PDB | External Database | Details |
---|---|---|
Ile 19 | Val 20 | conflict |
His 27 | Arg 28 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 108 |
Total formula weight | 4498518.4 | |
Non-Polymers* | Number of molecules | 1213 |
Total formula weight | 30301.1 | |
All* | Total formula weight | 4528819.4 |