5EHU

sfGFP mutant with unnatural amino acid 4-azidoethoxy-L-phenylalanine incorporated at the 149 site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Green fluorescent protein	polymer	239	27017.5	2	UniProt (A0A059PIQ0) Pfam (PF01353) UniProt (by SIFTS) (P42212) In PDB	Aequorea victoria (Jellyfish)
2		water	water		18.0	553	Chemie (HOH)

Sequence modifications

A, B: 2 - 238 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Met 0	-	expression tag
Val 1	-	expression tag
Ser 2	Arg 2	engineered mutation
Arg 30	Ser 30	engineered mutation
Cro 66	Thr 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 72	Ala 72	engineered mutation
Arg 80	Gln 80	engineered mutation
4lz 149	Asn 149	engineered mutation
Val 206	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54034.9
All*	Total formula weight	54034.9

*Water molecules are not included.