5EHT
Indirect contributions of mutations underlie optimization of new enzyme function
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | N-acyl homoserine lactonase | polymer | 253 | 28468.3 | 1 | UniProt (A3FJ64) Pfam (PF00753) | Bacillus thuringiensis | AHL-lactonase,Homoserine lactone lactonase |
| 2 | B, C (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 3 | D, E, F, G, H... (A) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 4 | J (A) | water | water | 18.0 | 247 | Chemie (HOH) |
Sequence modifications
A: 2 - 250 (UniProt: A3FJ64)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Val 9 | Ile 9 | engineered mutation |
| Phe 20 | Ser 20 | engineered mutation |
| Val 33 | Leu 33 | engineered mutation |
| Cso 64 | Phe 64 | engineered mutation |
| Gly 69 | Val 69 | engineered mutation |
| Thr 139 | Lys 139 | engineered mutation |
| Met 230 | Ile 230 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28468.3 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 683.4 | |
| All* | Total formula weight | 29151.7 |
