5E9Z
Cytochrome P450 BM3 mutant M11
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 469 | 53209.7 | 4 | UniProt (P14779) Pfam (PF00067) | Bacillus megaterium | Cytochrome P450(BM-3),Cytochrome P450BM-3 |
| 2 | E, I, K, M (A, B, C, D) | FE (II) ION | non-polymer | 55.8 | 4 | Chemie (FE2) | |||
| 3 | F, H, J, L (A, B, C, D) | PROTOPORPHYRIN IX | non-polymer | 562.7 | 4 | Chemie (PP9) | |||
| 4 | G (B) | 2,3-DIHYDROXY-1,4-DITHIOBUTANE | non-polymer | 154.3 | 1 | Chemie (DTT) | |||
| 5 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 1255 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 0 - 467 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Leu 47 | Arg 48 | engineered mutation |
| Gly 64 | Glu 65 | engineered mutation |
| Ile 81 | Phe 82 | engineered mutation |
| Val 87 | Phe 88 | engineered mutation |
| Gly 143 | Glu 144 | engineered mutation |
| Gln 188 | Leu 189 | engineered mutation |
| Cys 198 | Tyr 199 | engineered mutation |
| Val 267 | Glu 268 | engineered mutation |
| Tyr 285 | His 286 | engineered mutation |
| Ser 415 | Gly 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 212838.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2628.3 | |
| All* | Total formula weight | 215467.0 |
