5E65

The complex structure of Hemagglutinin-esterase-fusion mutant protein from the influenza D virus with receptor analog 9-O-Ac-3'SLN (Tr322)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Hemagglutinin-esterase	polymer	427	46457.0	2	UniProt (K9LG83) Pfam (PF03996) Pfam (PF02710) In PDB	Influenza D virus (D/swine/Oklahoma/1334/2011)
2	B, D	Hemagglutinin-esterase	polymer	166	17511.5	2	UniProt (K9LG83) Pfam (PF08720) Pfam (PF00509) In PDB	Influenza D virus (D/swine/Oklahoma/1334/2011)
3	E, F, J	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G94106MV)
4	G, I, K, L, N	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
5	H, M	9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonic acid-(2-3)-beta-D-galactopyranose	branched		513.4	2	In PDB GlyTouCan (G90454CH)
6	B, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
7		water	water		18.0	675	Chemie (HOH)

Sequence modifications

A, C: 3 - 429 (UniProt: K9LG83)

PDB	External Database	Details
Ala 57	Ser 73	engineered mutation
Ala 356	Asp 372	engineered mutation
Ala 359	His 375	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127937.0
Branched	Number of molecules	10
	Total formula weight	6367.8
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	134747.2

*Water molecules are not included.