5E63
K262A mutant of I-SmaMI
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | I-SmaMI LAGLIDADG meganuclease | polymer | 302 | 34094.5 | 1 | UniProt (F7WD42) Pfam (PF00961) | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
| 2 | B (B) | DNA right half site Bottom strand | polymer | 14 | 4248.8 | 1 | synthetic construct | ||
| 3 | C (C) | DNA left half site bottom strand | polymer | 11 | 3398.2 | 1 | synthetic construct | ||
| 4 | D (D) | DNA left site top strand | polymer | 15 | 4535.0 | 1 | synthetic construct | ||
| 5 | E (E) | DNA (5'-D(P*CP*AP*GP*GP*TP*GP*TP*AP*CP*G)-3') | polymer | 10 | 3085.0 | 1 | synthetic construct | ||
| 6 | F, G, H (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | I (A) | 2-METHOXYETHANOL | non-polymer | 76.1 | 1 | Chemie (MXE) | |||
| 8 | J (E) | 2-(2-METHOXYETHOXY)ETHANOL | non-polymer | 120.1 | 1 | Chemie (PG0) | |||
| 9 | K, L, M, N, O (A, B, C, D, E) | water | water | 18.0 | 49 | Chemie (HOH) |
Sequence modifications
A: 1 - 302 (UniProt: F7WD42)
| PDB | External Database | Details |
|---|---|---|
| Asn 165 | Leu 278 | conflict |
| Ala 262 | Lys 375 | engineered mutation |
| Gln 267 | Met 380 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 49361.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 269.2 | |
| All* | Total formula weight | 49630.7 |
