5E5S
I-SmaMI K103A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | I-SmaMI LAGLIDADG meganuclease | polymer | 302 | 34094.5 | 1 | UniProt (F7WD42) Pfam (PF00961) | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
| 2 | B (B) | Bottom strand DNA | polymer | 25 | 7692.0 | 1 | synthetic construct | ||
| 3 | C (C) | Top strand DNA left site | polymer | 15 | 4535.0 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*CP*AP*GP*GP*TP*GP*TP*AP*CP*G)-3') | polymer | 10 | 3085.0 | 1 | synthetic construct | ||
| 5 | E, F, G (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 6 | H (B) | 2-(2-METHOXYETHOXY)ETHANOL | non-polymer | 120.1 | 1 | Chemie (PG0) | |||
| 7 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 23 | Chemie (HOH) |
Sequence modifications
A: 1 - 302 (UniProt: F7WD42)
| PDB | External Database | Details |
|---|---|---|
| Ala 103 | Lys 216 | engineered mutation |
| Asn 165 | Leu 278 | conflict |
| Gln 267 | Met 380 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 49406.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 193.1 | |
| All* | Total formula weight | 49599.6 |
