5E35

Crystal structure of H5 hemagglutinin mutant (N224K, Q226L, N158D and L133a deletion) from the influenza virus A/chicken/Vietnam/NCVD-093/2008 (H5N1) with LSTc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hemagglutinin	polymer	333	37663.8	1	UniProt (C4P282) Pfam (PF00509) In PDB	Influenza A virus (A/chicken/Vietnam/NCVD-093/2008(H5N1))	HEMAGGLUTININ HA1 CHAIN
2	B	Hemagglutinin	polymer	180	20793.0	1	UniProt (C4P282) Pfam (PF00509) In PDB	Influenza A virus	HEMAGGLUTININ HA2 CHAIN
3	C	N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	1	In PDB BIRD (PRD_900046) GlyTouCan (G73578JC)		6'-sialyl-N-acetyllactosamine
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5		water	water		18.0	49	Chemie (HOH)

Sequence modifications

A: 11 - 333 (UniProt: C4P282)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag
-	Leu 145	deletion
Asp 158	Asn 170	engineered mutation
Lys 224	Asn 236	engineered mutation
Leu 226	Gln 238	engineered mutation

B: 1 - 175 (UniProt: C4P282)

PDB	External Database	Details
Arg 176	-	expression tag
Leu 177	-	expression tag
Val 178	-	expression tag
Pro 179	-	expression tag
Arg 180	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58456.8
Branched	Number of molecules	1
	Total formula weight	674.6
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	59573.9

*Water molecules are not included.