5E2Z

Crystal structure of H5 hemagglutinin Q226L mutant from the influenza virus A/duck/Egypt/10185SS/2010 (H5N1) with LSTa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, A, E	Hemagglutinin	polymer	333	37701.6	3	UniProt (G8IPF0) Pfam (PF00509) In PDB	Influenza A virus (A/duck/Egypt/10185SS/2010(H5N1))	HEMAGGLUTININ HA1 CHAIN
2	D, B, F	Hemagglutinin	polymer	180	20822.0	3	UniProt (G8IPF0) Pfam (PF00509) In PDB	Influenza A virus	HEMAGGLUTININ HA2 CHAIN
3	G, K	beta-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G46874CY)
4	H, L	N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose	branched		471.4	2	In PDB GlyTouCan (G30207PZ)
5	I	beta-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		716.7	1	In PDB GlyTouCan (G62810GW)
6	J	N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	1	In PDB GlyTouCan (G46776WR)
7	C, A, E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
8		water	water		18.0	167	Chemie (HOH)

Sequence modifications

C, A, E: 11 - 333 (UniProt: G8IPF0)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag
Leu 226	Gln 237	engineered mutation

D, B, F: 2 - 175 (UniProt: G8IPF0)

PDB	External Database	Details
Gly 1	-	expression tag
Arg 176	-	expression tag
Leu 177	-	expression tag
Val 178	-	expression tag
Pro 179	-	expression tag
Arg 180	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	175570.9
Branched	Number of molecules	6
	Total formula weight	3475.2
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	179709.7

*Water molecules are not included.