5DYJ

Mysosin heavy chain kinase A catalytic domain mutant - D663A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Myosin heavy chain kinase A	polymer	307	34518.4	2	UniProt (P42527) Pfam (PF02816) In PDB	Dictyostelium discoideum (Slime mold)	MHCK-A
2	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A, B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
6		water	water		18.0	101	Chemie (HOH)

Sequence modifications

A, B: 552 - 841 (UniProt: P42527)

PDB	External Database	Details
Met 535	-	initiating methionine
Gly 536	-	expression tag
Gly 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
Gly 544	-	expression tag
Glu 545	-	expression tag
Asn 546	-	expression tag
Leu 547	-	expression tag
Tyr 548	-	expression tag
Phe 549	-	expression tag
Gln 550	-	expression tag
Gly 551	-	expression tag
Ala 663	Asp 663	engineered mutation
Asn 818	Lys 818	conflict
Gln 819	Lys 819	conflict
Lys 828	Met 828	conflict
Ala 829	Pro 829	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69036.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1199.4
All*	Total formula weight	70236.1

*Water molecules are not included.