5DWK
Diacylglycerol Kinase solved by multi crystal multi orientation native SAD
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Diacylglycerol kinase | polymer | 130 | 14204.5 | 6 | UniProt (P0ABN1) Pfam (PF01219) | Escherichia coli | DAGK,Diglyceride kinase,DGK |
| 2 | G, H, I, K, L... (A, B, D) | (2R)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE | non-polymer | 314.5 | 7 | Chemie (78N) | |||
| 3 | J, N, P, Q, R... (A, B, C) | (2S)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE | non-polymer | 314.5 | 6 | Chemie (78M) | |||
| 4 | O (B) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | T (C) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | U (C) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 7 | V (C) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 8 | AA, BA, CA, X, Y... (D, E, F, A, B...) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 121 (UniProt: P0ABN1)
| PDB | External Database | Details |
|---|---|---|
| Gly -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Cys 41 | Ala 42 | engineered mutation |
| Ala 46 | Cys 47 | engineered mutation |
| Val 53 | Ile 54 | engineered mutation |
| Leu 70 | Ile 71 | engineered mutation |
| Leu 96 | Met 97 | engineered mutation |
| Asp 107 | Val 108 | engineered mutation |
| Ala 113 | Cys 114 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 85226.7 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 4427.5 | |
| All* | Total formula weight | 89654.3 |
