5DS6

Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA with splayed ends

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	CRISPR-associated endonuclease Cas1	polymer	306	33322.5	4	UniProt (Q46896) Pfam (PF01867) In PDB	Escherichia coli (strain K12)
2	E, F	CRISPR-associated endoribonuclease Cas2	polymer	104	11684.5	2	UniProt (P45956) Pfam (PF09707) In PDB	Escherichia coli (strain K12)
3	G	DNA (29-MER)	polymer	33	10180.6	1		Enterobacteria phage M13
4	H	DNA (28-MER)	polymer	33	10067.5	1		Enterobacteria phage M13

Sequence modifications

A, B, C, D: 1 - 305 (UniProt: Q46896)

PDB	External Database	Details
Ser 0	-	expression tag

E, F: 1 - 94 (UniProt: P45956)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 95	-	expression tag
Ser 96	-	expression tag
Ser 97	-	expression tag
Glu 98	-	expression tag
Asn 99	-	expression tag
Leu 100	-	expression tag
Tyr 101	-	expression tag
Phe 102	-	expression tag
Gln 103	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	176907.0
All*	Total formula weight	176907.0

*Water molecules are not included.