5DND

Crystal structure of the Asn-bound guinea pig L-asparaginase 1 catalytic domain active site mutant T116A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase	polymer	588	63354.7	4	UniProt (H0W0T5) Pfam (PF00710) Pfam (PF17763) Pfam (PF12796)	Cavia porcellus (Guinea pig)
2	E, F, H, J (A, B, C, D)	ASPARAGINE	non-polymer		132.1	4	Chemie (ASN)
3	G, I, K, L (B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
4	M, N, O, P (A, B, C, D)	water	water		18.0	399	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 565 (UniProt: H0W0T5)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Asn -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 116	Thr 116	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	253418.9
Non-Polymers*	Number of molecules	8
	Total formula weight	776.7
All*	Total formula weight	254195.6

*Water molecules are not included.