5DF8

CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH CEFOPERAZONE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cell division protein	polymer	564	61152.6	2	UniProt (Q51504) Pfam (PF03717) Pfam (PF00905)	Pseudomonas aeruginosa	Penicillin-binding protein 3,Pseudomonas aeruginosa genome assembly PAE221
2	C, J (A, B)	(2R,4R,5R)-2-[(1R)-1-{[(2R)-2-{[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino}-2-(4-hydroxyphenyl)acetyl]amino}-2-oxoethyl]-5-methyl-1,3-thiazinane-4-carboxylic acid	non-polymer		535.6	2	Chemie (59F)
3	D, E, F, K, L... (A, B)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	G, H, I, N, O... (A, B)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
5	Q, R (A, B)	water	water		18.0	598	Chemie (HOH)

Sequence modifications

A, B: 35 - 579 (UniProt: Q51504)

PDB	External Database	Details
Met 16	-	initiating methionine
Ala 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Ser 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Leu 27	-	expression tag
Glu 28	-	expression tag
Val 29	-	expression tag
Leu 30	-	expression tag
Phe 31	-	expression tag
Gln 32	-	expression tag
Gly 33	-	expression tag
Pro 34	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	122305.2
Non-Polymers*	Number of molecules	14
	Total formula weight	1836.4
All*	Total formula weight	124141.6

*Water molecules are not included.