5D9M
Crystal structure of PbGH5A, a glycoside hydrolase family 5 enzyme from Prevotella bryantii B14, E280A mutant in complex with the xyloglucan tetradecasaccharide XXXGXXXG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | B-1,4-endoglucanase | polymer | 353 | 39329.5 | 2 | UniProt (O06842) Pfam (PF00150) | Prevotella bryantii | |
| 2 | C (C) | alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1062.9 | 1 | In PDB GlyTouCan (G53479JR) | |||
| 3 | D (D) | alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 2107.8 | 1 | In PDB GlyTouCan (G16324DM) | |||
| 4 | E, F (A, B) | water | water | 18.0 | 728 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 353 (UniProt: O06842)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 280 | Glu 851 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 78658.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 3170.8 | |
| All* | Total formula weight | 81829.7 |
