5D49

Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Terminal deoxynucleotidyltransferase	polymer	400	45704.0	1	UniProt (Q3UZ80) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791) UniProt (by SIFTS) (P09838)	Mus musculus (Mouse)
2	B (B)	DNA (5'-D(*AP*AP*AP*AP*AP*C)-3')	polymer	6	1810.3	1		synthetic construct
3	C, D (C, D)	DNA (5'-D(*TP*TP*TP*TP*TP*GP*GP*C)-3')	polymer	8	2423.6	2		synthetic construct
4	E (E)	DNA (5'-D(*AP*AP*AP*AP*A)-3')	polymer	5	1521.1	1		synthetic construct
5	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	G (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	H, I (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
8	J, K, L (A)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
9	M (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
10	N, O, P, Q, R (A, B, C, D, E)	water	water		18.0	573	Chemie (HOH)

Sequence modifications

A: 132 - 510 (UniProt: Q3UZ80)

PDB	External Database	Details
Met 111	-	initiating methionine
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	53882.5
Non-Polymers*	Number of molecules	8
	Total formula weight	610.8
All*	Total formula weight	54493.3

*Water molecules are not included.