5D3G

Structure of HIV-1 Reverse Transcriptase Bound to a Novel 38-mer Hairpin Template-Primer DNA Aptamer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	HIV-1 REVERSE TRANSCRIPTASE P66 subunit	polymer	555	63875.1	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Pr160Gag-Pol
2	B, D (B, D)	HIV-1 REVERSE TRANSCRIPTASE P51 subunit	polymer	444	51928.6	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Pr160Gag-Pol
3	E, F (F, E)	DNA aptamer (38-MER)	polymer	38	11748.5	2		synthetic construct
4	G, H (G, H)	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)		sucrose
5	I, P (A, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	J, K, L, M, N... (A, B, C, D, F)	GLYCEROL	non-polymer		92.1	11	Chemie (GOL)
7	AA, V, W, X, Y... (E, A, B, C, D...)	water	water		18.0	447	Chemie (HOH)

Sequence modifications

A, C: 1 - 555 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation

B, D: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Met -15	-	initiating methionine
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	255104.5
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	13
	Total formula weight	1205.2
All*	Total formula weight	256994.3

*Water molecules are not included.