5D2H

4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - complexed with magnesium and alpha-ketoglutarate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	4-oxalocrotonate decarboxylase NahK	polymer	283	30746.1	1	UniProt (Q1XGK3) Pfam (PF01557) In PDB	Pseudomonas putida
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	2-OXOGLUTARIC ACID	non-polymer		146.1	1	Chemie (AKG)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
8		water	water		18.0	270	Chemie (HOH)

Sequence modifications

A: 1 - 264 (UniProt: Q1XGK3)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Pro 155	Leu 155	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30746.1
Non-Polymers*	Number of molecules	14
	Total formula weight	919.6
All*	Total formula weight	31665.7

*Water molecules are not included.