5D0K

Structure of UbE2D2:RNF165:Ub complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, D, G, J)	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17144.5	4	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B, D, F, H (C, F, I, L)	RING finger protein 165	polymer	92	10360.9	4	UniProt (Q6ZSG1) Pfam (PF13639)	Homo sapiens (Human)
3	I, J, K, L (B, E, H, K)	Polyubiquitin-B	polymer	76	8576.8	4	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
4	M, N, O, P, Q... (C, F, I, L)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
5	U, V, W (L, E, H)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A, D, G, J: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

C, F, I, L: 255 - 346 (UniProt: Q6ZSG1)

PDB	External Database	Details
Gly 250	-	expression tag
Pro 251	-	expression tag
Leu 252	-	expression tag
Gly 253	-	expression tag
Ser 254	-	expression tag
-	Arg 273	deletion
-	Arg 274	deletion
-	Pro 275	deletion
-	Gln 276	deletion
-	Asp 277	deletion

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	144328.9
Non-Polymers*	Number of molecules	8
	Total formula weight	523.3
All*	Total formula weight	144852.1

*Water molecules are not included.