5CZX

Crystal structure of Notch3 NRR in complex with 20358 Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Neurogenic locus notch homolog protein 3	polymer	271	30096.6	2	UniProt (Q9UM47) Pfam (PF00066) Pfam (PF06816) Pfam (PF07684)	Homo sapiens (Human)	Notch 3
2	C, E (C, H)	20358 Fab heavy chain	polymer	226	24118.0	2		Homo sapiens (Human)	IgG20358 heavy chain, NVP-LMW985 heavy chain
3	D, F (D, L)	20358 Fab light chain	polymer	214	23387.9	2		Homo sapiens (Human)	IgG20358 light chain, NVP-LMW985 light chain
4	G, N (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	H, I, J, O, P... (A, B)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
6	AA, K, L, M, R... (L, A, B, C, D...)	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
7	T, X, Z (C, H, L)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8	BA, CA, DA, EA, FA... (A, B, C, D, H...)	water	water		18.0	850	Chemie (HOH)

Sequence modifications

A, B: 1378 - 1640 (UniProt: Q9UM47)

PDB	External Database	Details
Gly 1641	-	expression tag
Ser 1642	-	expression tag
His 1643	-	expression tag
His 1644	-	expression tag
His 1645	-	expression tag
His 1646	-	expression tag
His 1647	-	expression tag
His 1648	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	155205.0
Non-Polymers*	Number of molecules	21
	Total formula weight	1409.9
All*	Total formula weight	156615.0

*Water molecules are not included.