5CHZ

Structure of wild-type human MBD4 bound to a G:T mismatch

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methyl-CpG-binding domain protein 4	polymer	192	22487.8	1	UniProt (O95243)	Homo sapiens (Human)	Methyl-CpG-binding endonuclease 1,Methyl-CpG-binding protein MBD4,Mismatch-specific DNA N-glycosylase
2	B (C)	7-mer DNA	polymer	7	2098.4	1		synthetic construct
3	C (D)	12-mer DNA(G)	polymer	12	3695.4	1		synthetic construct
4	D (B)	5-mer DNA	polymer	5	1505.0	1		synthetic construct
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F, G, H, I (A, C, D, B)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A: 426 - 580 (UniProt: O95243)

PDB	External Database	Details
Met 389	-	initiating methionine
Gly 390	-	expression tag
Ser 391	-	expression tag
Ser 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
Ser 399	-	expression tag
Ser 400	-	expression tag
Gly 401	-	expression tag
Leu 402	-	expression tag
Val 403	-	expression tag
Pro 404	-	expression tag
Arg 405	-	expression tag
Gly 406	-	expression tag
Ser 407	-	expression tag
His 408	-	expression tag
Met 409	-	expression tag
Ala 410	-	expression tag
Ser 411	-	expression tag
Met 412	-	expression tag
Thr 413	-	expression tag
Gly 414	-	expression tag
Gly 415	-	expression tag
Gln 416	-	expression tag
Gln 417	-	expression tag
Met 418	-	expression tag
Gly 419	-	expression tag
Arg 420	-	expression tag
Gly 421	-	expression tag
Ser 422	-	expression tag
Glu 423	-	expression tag
Phe 424	-	expression tag
Met 425	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	29786.6
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	29811.0

*Water molecules are not included.